Seven different species of Aspergillus, A. niger, A. oryzae, A. sojae, A. tamari, A. nidulins, A. flavus, and A. parasiticus were available to observe microscopically, the differences in the morphology within the species. Demonstrations of various ways to set up Riddell mounts were described. Riddell mounts are utilized to examine fungi microscopically to minimalize the disturbance of various characteristic fungal structures, ie. conidiophores, vesicles, phialides, metulae, conidia.
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| Riddell mount with agar block cut directly from A. flavus culture |
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| Variant of Riddell mount with inoculated agar block and an agar block directly from A. niger culture |
Aspergillus sp. can be either bisereate and/or unisereate.
Selective media was discussed in order to isolate unknown fungi. Water agar (encourages conidial growth, lacking nutients), Rose Bengal agar (has antimicrobial properties), Potato Dextrose agar (PDA), Cornmeal Agar (CMA), 1/2 strenght PDA (encourages conidial growth while providing some nutrients)+ Ampicillin (to discourage bacterial growth).
Sub-culturing was briefly discussed.
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