Lab Summary 10/3/12

1.  The following cultures were available for microscopic examination to aid in unknown identifications and the various different spore types, condiogenous cells, and hyphae.

Alternaria brassicicola, Aspergillus niger, Botrytis cinera, Colletotrichum coccodes, Curvularia sp., Epicoccum sp., Fusarium graminearum, Monilinia fructicola, Nigrospora sp., Pestilotia sp., Rhizoctonia solani, Thielaviopsis brasicola, and Trichoderma viride. 

Fusarium graminearum  macroconidia and microconidia

Rhizoctonia solani hypae with septation

 

 

 

 

 

Pigmented Pestilotia sp spores

Thielaviopsis brasicola in pansy roots (TPDDL sample) with aleuriospores 

 

 

 Alternaria brassicicola pigmented spores with cross and longitudinal septa

Neurospora crassa cross resulting
in the formation of Perithecia

2.  Neurospora crassa crosses (SmRP10 x SmRP 11, SmRP10 x CP-1 GFP, SmPR10 x NCACo21-1) were successful.  Perithicia were present however, the ostioles had not yet formed.  It was observed in some of the agar plates trichogynes (specialized hypae) of N. crassa were seeking the opposite mating type across the plate.

















 3.  The week before (9/26/12) 2 different corn varieties (Silver Queen and OPR2-1) were inoculated with the haploid strain of Utilago maydis Sg200.  A week later we observed tumor formation and proceeded to prepare the tissue for fungal observation.  Proceedure as follows:  Destain plant tissue with 2:1 acetic acid:ethanol for 24 hours, stain with trifan blue, and then briefly destain.

Detatining (24hr) of the corn leaf sections inoculated with Ustilago maydis
showing symptom expression (tumors)

4.  A week (inoculated on 9/26/12) after the dimorphic experiment was set up, all but one of the Mucor rouxii cups were contaminated with N. crassa and were slightly dried out.  I took a cross sections through the agar and did observe the two different stages via compound microscopy, the yeast stage and the filamentous stage (hyphae).